Antioxidant report

White Paper on In-Vitro Bioactivity of ASEA™ Related to Toxicity, Glutathione Peroxidase, Superoxide Dismutase Efficacy and Related Transcription Factors by Dr. G. Samuelson – June 22, 2010

“Results of Further Investigations into SOD enzymatic activity at low ASEA concentrations (< 1%): It was found in another investigation that NRF2 nuclear translocation (data and results are in the following sections), took place at low doses of ASEA (less than 1%) and elicited peak SOD antioxidant activity at about 30 to 120 minutes after exposure. Thus when SOD antioxidant activity was measured due to low-concentration ASEA exposure at a 30 to 120 minute time points, results similar to the GPx enzymatic activity were seen both with murine epidermal (JB6) cells and serum-starved HMVEC-L cells at a time point 90 to 120 minutes. Graphs were not supplied, however, a 500% increase in peak SOD enzymatic activity was estimated over a short 120 minute term, with 95% confidence. Specific work on low-level concentration dependence still has yet to be done.

Brief Summary of Results for Objective (2): An 800% increase in GPx antioxidant efficacy in HMVEC-L cells was seen after 24 hours exposure from low-concentration ASEA (no concentration dependence seen). A transitory increase of up to 500% was seen in SOD antioxidant efficacy between 30 to 90 min. Again after exposure to low-concentration ASEA (< 1%). In both cases, the low concentrations of ASEA were comparable to blood concentrations possible from oral dosing, though data is not available to confirm this. Concentration dependence at very low concentrations might be seen if such was carefully investigated. Exposure to high-concentration ASEA, in comparison, elicited only a small relative increase in GPx antioxidant efficacy that was not concentration dependent. An increase in SOD efficacy was not seen for either high-concentration ASEA or after long (24 hr) exposures. In subsequent investigations, this information will be used to determine optimal concentrations and time points to study concentration dependence (less than 0.1% and 0-120 minutes).

Interpretations and Conclusions The infusion of a certain balanced mixture of redox signalling molecules, ASEA, into viable HMVEC-L and JB6 cell cultures has been seen to elicit distinct bioactivity. No indications of toxicity or the expression of inflammatory cytokines were observed and yet there was increased antioxidant and protective enzyme expression (as evidenced by increased nuclear NRF2) and greatly increased efficacy for the two master antioxidants, GPx and SOD.”

From Dr. G. Samuelson’s pdf below. He is the gentleman who stabilised the redox molecules.

Results for Antioxidant Up‐Regulation

“The results for these tests were extraordinary in several regards.  First, there was a real up‐regulation of anti‐oxidant production in cells exposed to ASEA. This effect was temporary, lasting only about 120 minutes but was clearly visible.  The most interesting result, however, is that exposure to ASEA at any concentration did not invoke the normal inflammatory transcription factor (NF‐kappaB) and yet did invoke the antioxidant transcription factor (NRF2).   Stimulating the production of antioxidants without stimulation of low‐level inflammation is very rare and has stirred some interest in the scientific community.”

My eternal gratitude to him and all the team behind this wonderful compassionate technology, that has been helping me tremendously and thousands of others across the globe.